Optimizing in vitro Growth Conditions for Magnolia 'Ann'
نویسندگان
چکیده
Significance to Industry: Magnolia (M. liliiflora ‘Nigra’× M. stellata ‘Rosea’) ‘Ann’ (NA 28344; PI 326570) is a member of the ‘Little Girl’ series of magnolias that have become widely popular. Magnolia ‘Ann’ is characterized by a desirable combination of traits including prolific and remontant flowering and a shrubby form. Due to difficulties in propagating magnolias from cuttings or grafting, development and optimization of in vitro propagation methods would be desirable. Magnolia ‘Ann’ is also a triploid, interspecific hybrid that is reportedly sterile (22). In vitro chromosome doubling may be an approach to develop allopolyploids with restored fertility (4) and provide an opportunity to use this cultivar in future breeding programs. To enhance the ornamental qualities of ‘Ann’, micropropagation protocols were developed as a platform for propagation and future ploidy level manipulation. Murashige and Skoog basal medium (MS)(17) supplemented with 2 μM benzylamino purine (BAP) provided high shoot proliferation, while Lloyd and McCown Woody Plant Medium (WPM)(8) containing charcoal may be used to produce elongated plantlets more suitable for rooting and ex vitro establishment.
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